Study of hepatoprotective properties of protein fractions obtained from leaves of Tagetes erecta L. and Salvia aethiopis L. plants by gel filtration chromatography on tetrachloromethane (CCl4) damaged hepatocytes of mice
DOI:
https://doi.org/10.56580/GEOMEDI47Keywords:
Tagetes erecta L., Salvia Aethiopis L., Imeretian saffron, proteins, gel filtration chromatography, ion exchange chromatography, tetrachloromethane, CCl4, gel electrophoresis, histomorphology, hepatocytesAbstract
The study presents data on the action of protein fractions obtained from Tagetes erecta L. and Salvia aethiopis L. plants by gel filtration chromatography on the hepatocytes of mice damaged by a 10% tetrachloromethane solution. Protein fractions of the third and fourth peaks have weak hepatoprotective properties. Each peak was also separated into several fractions by the method of ion exchange chromatography. The SDS-PAGE revealed fractions, including those with a molecular weight of 23-47 kDa, which probably participate in the protection of hepatocytes.
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Hinojosa Espinosa, Oscar; Schiavinato, Dario Javier; Phylogeny of marigolds (Tagetes L., Tageteae) based on ITS sequences; The International Compositae Alliance; Capitulum; 2; 8-2022; 38-49
Salehi, Bahare, Marco Valussi, Maria Flaviana Bezerra Morais-Braga, Joara Nalyda Pereira Carneiro, Antonio Linkoln Alves Borges Leal, Henrique Douglas Melo Coutinho, Sara Vitalini, Dorota Kręgiel, Hubert Antolak, Mehdi Sharifi-Rad, and et al. 2018. "Tagetes spp. Essential Oils and Other Extracts: Chemical Characterization and Biological Activity" Molecules 23, no. 11: 2847. https://doi.org/10.3390/molecules23112847
Rodov V., Vinokur T., Gogia N., Chkhikvishvilli I.D. Hydrophilic and Lypophilic Antioxidant Capacities of Georgian Spices for Meat and Their Possible Health Implication. Georgian Medical News. №2(179)2010.
Zanovello M, Bolda Mariano LN, Cechinel-Zanchett CC, Boeing T, Tazinaffo GC, Mota da Silva L, Silva DB, Gasparotto Junior A, de Souza P. Tagetes erecta L. flowers, a medicinal plant traditionally used to promote diuresis, induced diuretic and natriuretic effects in normotensive and hypertensive rats. J Ethnopharmacol. 2021 Oct 28;279: 114393. doi: 10.1016/j.jep. 114393. Epub 2021 Jul 3. PMID: 34229058.
Li-wei XU, Juan CHEN, Huan-yang QI, Yan-ping SHI, Phytochemicals and Their Biological Activities of Plants in Tagetes L., Chinese Herbal Medicines, Volume 4, Issue 2, 2012, Pages 103-117, ISSN 1674-6384,
https://doi.org/10.3969/j.issn.1674-6384. 2012.02.004.
Marı́a L Tereschuk, Marta V.Q Riera, Guillermo R Castro, Lidia R Abdala, Antimicrobial activity of flavonoids from leaves of Tagetes minuta, Journal of Ethnopharmacology, Volume 56, Issue 3,1997,Pages 227-232,ISSN 0378-8741, https://doi.org/10.1016/S0378-8741(97) 00038-X.
Kashif, M., Bano, S., Naqvi, S., Faizi, S., Lubna, Ahmed Mesaik, M., … Farooq, A. D. (2014). Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower. flower. Pharmaceutical Biology, 53(5), 672–681. https://doi.org/10.3109/13880209.2014.936471
Dunkel FV, Jaronski ST, Sedlak CW, Meiler SU, Veo KD. Effects of steam-distilled shoot extract of Tagetes minuta Asterales: (Asteraceae) and entomopathogenic fungi on larval Tetanops myopaeformis. Environ Entomol. 2010 Jun;39(3):979-88. doi: 10.1603/EN09259. PMID: 20550813.
Mares D, Tosi B, Poli F, Andreotti E, Romagnoli C. Antifungal activity of Tagetes patula extracts on some phytopathogenic fungi: ultrastructural evidence on Pythium ultimum. Microbi- ol Res. 2004;159(3):295-304. doi: 10. 1016 /j.micres.2004.06.001. PMID: 15462529.
Korkotadze T, Berashvili D, Mshvildadze V, Getia M, Bakuradze A. Genus Salvia L. Study of Same Species, Growing in Georgia, Chemical Composition and Antioxidant Activity.
Journal of Experimental and Clinical Medicine Print-ISSN 1512-0392; E-ISSN 2667-9736. № 5-6. 2021. (In Georgian).
Bechkri S, Alabdul Magid A, Voutquenne-Nazabadioko L, Berrehal D, Kabouche A, Lehbili M, Lakhal H, Abedini A, Gangloff SC, Morjani H, Kabouche Z. Triterpenes from Salvia argentea var. aurasiaca and their antibacterial and cytotoxic activities. Fitoterapia. 2019 Nov; 139:104296. doi: 10.1016/j.fitote. 2019. 104296. Epub 2019 Aug 8. PMID: 31401222.
Yi-Bing Wu, Zhi-Yu Ni, Qing-Wen Shi, Mei Dong, Hiromasa Kiyota, Yu-Cheng Gu, and Bin Cong Chemical Reviers 2012 112 (11), 5967-6026 DOI: 10.1021/cr200058f
Zare S, Mirkhani H, Firuzi O, Moheimanian N, Asadollahi M, Pirhadi S, Chandran JN, Schneider B, Jassbi AR. Antidiabetic and cytotoxic polyhydro- xylated oleanane and ursane type triterpenoids from Salvia grossheimii. Bioorg Chem. 2020 Nov; 104:104297. doi: 10.1016/j.bioorg. 2020. 104297. Epub 2020 Sep 19. PMID: 33011536.
Hafsa Chouit1, Ouassila Touafek1*, Moussa Brada2, Chawki Benssouici3, Marie-Laure Fauconnier4, Mohamed El Hattab1 GC-MS Analysis and Biological Activities of Algerian Salvia microphylla Essential OilsJ. Mex. Chem. Soc.2021, 65(4) Regular Issue © 2021, Sociedad Química de México ISSN-e 2594-0317
Ayoub IM, George MY, Menze ET, Mahmoud M, Botros M, Essam M, Ashmawy I, Shendi P, Hany A, Galal M, Ayman M, Labib RM. Insights into the neuroprotective effects of Salvia officinalis L. and Salvia microphylla Kunth in the memory impairment rat model. Food Funct. 2022 Feb 21;13 (4):2253-2268. doi: 10.1039/d1fo02988f. PMID: 35137748.
O.H. Lowry, N.J. Rosenbrought, A.L. Far, R.J. Randall (1951), J. Biol. Chem., 105: 1-5.
Bouchardy B, Majno G. Histopathology of early myocardial infarcts. A new approach. Am J Pathol. 1974 Feb;74(2):301-30. PMID: 4359735; PMCID: PMC1910768.
Al Amin ASM, Menezes RG. Carbon Tetrachloride Toxicity. 2023 Sep 4. In: StarPearls [Internet]. Treasure Island I (FL): StatPearls Publishing; 2024 Jan–. PMID: 32965851.
Wang S, Friedman SL. Found in translation-Fibrosis in metabolic dysfunction-associated steatohepatitis (MASH). Sci Transl Med. 2023 Oct 4;15 (716):eadi0759. doi: 10.1126/ scitranslmed. adi0759. Epub 2023 Oct 4. PMID: 37792957; PMCID: PMC10671253.
